Home > Sandwich ELISA Kits > Human Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta isoform (PIK3Cb) ELISA Kit

Product Name:

Human Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta isoform (PIK3Cb) ELISA Kit

Cat.#:

JL18221

Regulatory Status:

RUO

Alternative:

PI3K; PI3Kbeta; PIK3C1; p110-BETA; Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit beta；Phosphoinositide-3-Kinase Catalytic Beta Polypeptide

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.156-10ng/mL

Sensitivity:

0.08ng/mL

Specificity:

This kit recognizes Human PIK3Cb in samples. No significant cross-reactivity or interference between Human PIK3Cb and analogues was observed.

Sample Type:

Tissue homogenates, cell lysates and other biological fluids

Assay Procedure:

Research Area:

Signal Transduction

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| PIK3Cb

Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta isoform is an enzyme that is encoded by the PIK3CB gene.Phosphoinositide 3-kinases (PI3Ks) phosphorylate the 3-prime OH position of the inositol ring of inositol lipids. They have been implicated as participants in signaling pathways regulating cell growth by virtue of their activation in response to various mitogenic stimuli. PI3Ks are composed of a 110-kD catalytic subunit, such as PIK3CB, and an 85-kD adaptor subunit.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

Concentration (ng/mL)	10	5	2.5	1.25	0.625	0.312	0.156	0
OD	2.41	1.84	1.21	0.85	0.52	0.33	0.21	0.08
Corrected OD	2.33	1.76	1.13	0.77	0.44	0.25	0.13	-

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Human PIK3Cb spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Tissue Homogenates(n=8)	91-105	102
Cell Lysates(n=8)	96-108	103

| Linearity

Samples were spiked with high concentrations of Human PIK3Cb and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Tissue Homogenates(n=4)	Cell Lysates(n=4)
1 : 2	Range (%)	86-96	90-110
1 : 2	Average (%)	93	96
1 : 4	Range (%)	87-108	105-115
1 : 4	Average (%)	98	110